Obtaining of transgenic papaya plants var. Maradol roja that carry out the rice oryzacystatin gene

Milady F. Mendoza; Ana Luisa Darias; Maylín Cruz; Jorge Gallardo; Marisol Tejeda; Rafael Gómez; Orelvis Portal

Obtaining of transgenic papaya plants var. Maradol roja that carry out the rice oryzacystatin gene

2004

Papaya (Carica papaya L.), is severely affected by Papaya Ringspot virus, which belongs to plant potyvirus group. A recent strategy for pest control produced by this virus is the transformation with genes encoding cysteine proteinase inhibitors. Rice oryzacistatin gene encoding for cystatins, was inserted in a pCAMBIA binary vector, for genetic transformation of papaya somatic embryos var. Maradol roja, mediated by gene gun. Gene integration was confirmed by means of polimerase chain reaction using the primers designed from gene bar sequence. Forty out of eighty in vitro transgenic papaya lines amplified a 402 fragment which correspond to the expecting size.Key words: Carica papaya, genetic engineering, potyvirus, proteinase inhibitor

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