Moringa Oleifera Leaf Meal (MOLM) with Cocktail Enzyme Supplementation on Immunity, Serum biochemical and Antioxidant Activity of Layers

The objective of the present study was to evaluate the effect of Moringa oleifera leaf meal on immunity status, antioxidant and serum biochemical parameters of layers. Two hundred, 34-week-old BV 300 White Leghorn layer birds were randomly allotted to 50 replicates with 4 birds in each replicate and these replicates were in turn allotted to 5 dietary groups. T1 (Control) = Corn-soybean meal Basal diet (BD); T2= 5% MOLM in BD diet without enzyme supplementation; T3= 7.5% MOLM without enzyme supplementation; T4= 10% MOLM in BD diet without enzyme supplementation; T5= 10% MOLM with enzyme supplementation. The results revealed that the cell mediated immune response was significantly (P<0.01) higher in T5 when compared to other treatments, whereas no significant difference observed in the Humoral immune response among the birds fed diets with or without inclusion of moringa leaf meal. There was no significant difference was recorded in protein values among T1, T2 and T5, however, lower protein values were observed in T3 and T4 but not significantly differ with T1. There was no significant difference in albumin content among the treatments. Lower (P<0.01) cholesterol level was noticed in T5 and higher (P<0.01) cholesterol level was observed in T1. The calcium and phosphorus levels in serum were higher (P<0.01) in T4 and T5 as compared to other treatments, with the lowest (P<0.01) calcium and phosphorus levels recorded in T1. The glutathione peroxidase activity was significantly higher (P<0.01) in the birds fed Moringa leaf meal included diets as compared to the control. The lipid peroxidation and RBC catalase activity was inversely proportional to inclusion levels of MOLM in diets. Thus, 10% Moringa oleifera leaf meal with or without enzyme supplementation could be used as improving immunity and antioxidant status of layers.