Bioloģiski aktīvu savienojumu noteikšana augu eļļās ar viedtālrunī iegūtu attēlu analīzi | Determination of biologically active compounds in vegetable oils by smartphone image analysis
2021
Vucane, S., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia). Faculty of Food Technology | Cinkmanis, I., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia). Faculty of Food Technology | Sabovics, M., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia). Faculty of Food Technology
Vegetable oils contain biologically active compounds that can be detected using a smartphone as an alternative to classical optical analytical methods. One of such vegetable oils of biologically active compounds are antioxidants, which inhibit the oxidation of fats, prolonging the shelf life and preserving the original properties of the sensors. Antioxidants react with radicals in vegetable oils to form new, more stable radicals that have oxidation reactions that are slowed down. To determine the antiradical activity of vegetable oils, a method based on 2.2–biphenyl–1–picryl hydrazil, or DPPH reagent, is proposed. Huawei P30 lite smartphone was used for image analysis. The free radical scavenging activity was detected by using Android application “ColorPicker” with the image matching algorithm for RGB model. Chromatography vials, PS cuvettes (PSK) and UV/Vis spectrophotometer was used for data comparison. Eleven vegetable oils: sea buckthorn, sunflower, rice, macadamia nut, hemp, corn, grape, linseed, rapeseed, olive and milk thistle oils were selected for the analysis. The aim of the study is to determine whether it is possible to determine antiradical activity using glass chromatography vials compared to polystyrene cells with a smartphone image analysis. Based on the smartphone-based image analysis, the results showed that RG colour values can be determined in RSA vegetable oils by chromatography vials with the same accuracy as PSK.
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