Characterization of vitamin Bsub(12) compounds in the wild edible mushrooms black trumpet (Craterellus cornucopioides) and golden chanterelle (Cantharellus cibarius)
2012
Watanabe, F. (Tottori University, Tottori (Japan). School of Agricultural, Biological and Environmental Sciences) | Schwarz, J. | Takenaka, S. | Miyamoto, E. | Ohishi, N. | Nelle, E. | Hochstrasser, R. | Yabuta, Y.
This study determined the vitamin Bsub(12) content of six wild edible mushrooms which are consumed by European vegetarians. Zero or trace levels (0.01-0.09 microg/100 g dry weight) of vitamin Bsub(12) were determined in porcini mushrooms (Boletus spp.), parasol mushrooms (Macrolepiota procera), oyster mushrooms (Pleurotus ostreatus), and black morels (Morchella conica). By contrast, black trumpet (Craterellus cornucopioides) and golden chanterelle (Cantharellus cibarius) mushrooms contained considerable levels (1.09-2.65 microg/100 g dry weight) of vitamin Bsub(12). To determine whether C. cornucopioides or C. cibarius contained vitamin Bsub(12) or other corrinoid compounds that are inactive in humans, we purified a corrinoid compound using an immunoaffinity column and identified it as vitamin Bsub(12) based on LC/ESI-MS/MS chromatograms.
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