Enzymatic production of xylooligosaccharides from beechwood xylan: effect of xylanase preparation on carbohydrate profile of the hydrolysates
2019
Guido, E. S. | Silveira, J. T. | Kalil, S. J.
Xylooligosaccharides (XOS) are non-digestible food ingredients with beneficial effects on human health. In the present work, three different xylanase preparations from filamentous fungi were characterised and used in the enzymatic conversion of beechwood xylan into XOS. The degree of polymerisation (DP) and yield of XOS, besides the release of xylose in the hydrolysate, are influenced by the xylanolytic activities of xylanase preparation and biochemical properties of endo-1,4-β-xylanases. Enzymatic hydrolysis of beechwood xylan using β-xylosidase-free xylanase from Thermomyces lanuginosus produced more than 90% (w/v) XOS in the hydrolysate, equivalent to 9.18 mg/mL in 24 h of reaction time. The XOS obtained in highest amounts were xylobiose (DP 2, 66.46%) followed by xylotriose (DP 3, 25.10%), and a small amount of xylose (4.97%) was simultaneously obtained. These findings suggest that this type of xylanase preparation is very promising for obtaining high-pure XOS with lower DP in food industries.
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