Phytosterol accumulation as defense metabolites in oil palm - Ganoderma boninense pathogenic interaction
2011
Nusaibah S. A. | Siti Nor Akmar A. | Idris A. S. | Sariah M.
. This study shows that the interaction between G. boninense and oil palm produced many secondary metabolites including sterol compounds revealed by gas chromatography-mass spectrometry (GC-MS) analysis. The tolerant and susceptible oil palm seedlings were artificially infected with G. boninense and subjected to metabolite extraction. A total of 13 sterol compounds were identified from the root extracts of both tolerant and susceptible oil palm seedlings. The main sterol compounds identified were sitosterol, stigmasterol, campesterol and 7-ergostenol. The GC-MS library, namely NIST 08, Wiley 229 and comparison of fragmentation patterns of the mass spectra reported in literature made it possible to identify the sterol components present in the root extracts. The results indicate that the number and level of sterol compounds induced in infected oil palms were significantly higher than in uninfected oil palm seedlings. Variations in the type and level of compounds detected were also observed between infected tolerant and susceptible oil palm seedlings. This study provides information that relates sterol compounds to the oil palm defence mechanism against G. boninense.
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