Molecular variation among populations of two red spider mites, Tetranychus cinnabarinus (Boisduval) and T. kanzawai Kishida
2019
Laquinta, J.F. | Cayabyab, B.F. | Ardez, K.P. | de Roxas, M.dL. | Kahayon, C.A. | Guerrero, M.S.
Red spider mites (RSM) are considered as one of the major and widely distributed arthropod pests of cassava. These arthropods cause the sporadic drying out of the leaf that starts from the middle part spreading through the leaf blades sparing only the leaf veins. They usually attack mature leaves, but during very high infestation when leaves dry out easily, young leaves and shoots can also be attacked leading to stunted growth or even death of young leaves and shoots can also be attacked leading to stunted growth or even death of young planta. Gabriel's 1997 list included two morphologically-identified species of red spider mites infesting cassava in the Philippines, namely: Tetranychus cinnabarinus (Boisdual) and T. kanzawai Kishida. To aid an immediate and accurate species identification of these minute arthropods from collected samples in five provinces, DNA barcoding was conducted. Genomic DNA of each specimen were isolated and gene amplification of cytochrome oxidase I (COI), commonly used genetic marker for arthropods, was done through polymerase chain reaction (PCR). BLASTn alignments resulted to two putatively identified red spider mite species from five provinces. Molecular characterization of interspecific and intraspecific sample sequences with 879 bp were analyzed. Two polymorphic nucleotides at 665th and 668th positions (transition of T left-right arrow C) were very distinct among the species with various polymorphisms were found among local populations (Provinces) of each species. DNA barcodes were already deposited in the GenBank, which can be accessed by the public as soon as results are published.
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