Partially purified fructosyltransferase for production of low-glycemic index natural sweetener
2018
Acuña, N.A.S. | Matel, H.L.
There is a strong need to develop technologies for production of low glycemic index sweeteners to reverse the increasing prevalence of diabetes in the country, which is correlated with high consumption of sugar. Efforts to synthesize fructans using enzymes were explored. A fructosyltransferase was partially purified from a novel fungal isolate by ammonium sulfate precipitation. Such enzyme was subjected to various assays to characterize and evaluate the potential use for production of low-glycemic index natural sweeteners like fructooligosaccharides or FOS. Reaction was monitored for five days and the reaction products quantified through High Pressure Liquid Chromatography with Refractive Index detector using InertSustain NH2 column and acetonitrile-water (4:1; v/v). Assay using sucrose solution as a substrate demonstrates the evidence of 1-SST and 1-FFT activity by being able to produce 1-kestose and nystose, respectively. The partially purified fructosyltransferase has a specific activity of 0.38 U/mg and after 24 hours, the total fructooligosaccharide production reached 43.61 g/L. The results imply potential application for production of FOS.
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