Assessment of buffalo semen preservability upon using tris-egg yolk extender enriched with different concentrations of taurine
2021
Islam El-Sayed El-Seadawy(National Research Centre, Cairo (Egypt). Department of Animal Reproduction and Artificial Insemination) | Mohamed Ismail Shahba(National Research Centre, Cairo (Egypt). Department of Animal Reproduction and Artificial Insemination) | Reda Ibrahim El-Sheshtawy(National Research Centre, Cairo (Egypt). Department of Animal Reproduction and Artificial Insemination) | Walid Said El-Nattat(National Research Centre, Cairo (Egypt). Department of Animal Reproduction and Artificial Insemination) | Gamal Attia El-Sisy(National Research Centre, Cairo (Egypt). Department of Animal Reproduction and Artificial Insemination)
Cryopreservation is connected with increased undesirable premature sperm capacitation, reduced lifespan and fertility results. Taurine acts as an antioxidant improving the semen quality after cryopreservation. The objective of the current study is to assess the effect of addition of different levels of taurine amino acid to tris-extender on bull sperm cryo-preservability. Methods: The collected semen samples were pooled and diluted with tris-citric acid-fructose egg yolk extender (control, 0 percent taurine) and different concentrations of taurine (10 mM, 20 Mm, 30 Mm, 40 Mm, 50 Mm, 60 Mm, 70 Mm, 80 Mm, 90 Mm and 100 mM) to final concentration of 60 *10**(6) motile sperm/ml. Diluted semen was processed for freezing and evaluated for sperm parameters. Results: Data showed that taurine enriched extender improved sperm parameters during cooling. Post thawing sperm motility percent, membrane integrity percent, Alive sperm percent improved and the percentages of abnormal sperm was lowered upon using taurine enriched extender. Conclusion: It could be concluded that, most the concentrations of taurine in Tris-citrate egg yolk extender ameliorated the post cooling and post freezing semen quality in buffalo bulls.
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