Cd2+ and Pb2+ Induced Structural, Functional and Compositional Changes in The Liver and Muscle Tissue of Crucian Carp (Carassius auratus gibelio): an FT-IR Study
2017
Khan, Sher Ali | Liu, Xiaoyu | Li, Hong | Li, Jingna | Zhou, Peiyuan | Rehman, Zia ur | Khan, Rehman Ullah
Contamination of aquatic ecosystems with toxic metals such as Cd2+ and Pb2+ is a serious issue in the industrializedworld, which can affect freshwater fish even at low concentrations. The aim of the present study was to investigate the effectof Cd2+ and Pb2+ alone or in combination on the biochemical constituents of liver and muscle in Crucian carp using FourierTransform Infrared Spectroscopy (FT-IR). Results from the spectral analysis revealed significant decline in protein andincrease in lipids in the two tissues with marked effect caused by the combined exposure of Cd2+ and Pb2+. In case of liver,alteration in the intensity and band area at amide I resulted in a differential response of structural protein for the exposedgroups. Furthermore, a decrease in the α-helix and alterations in the nucleic acids content was also observed in both liver and muscle of the exposed fish. Moreover, biochemical alterations in the vital tissue of freshwater fish due to toxic contaminants can be used as a marker of environmental pollution with the help of FT-IR spectroscopy.
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