Determination of organic acid and polyphenol content in grape seeds obtained with pressurized water extraction
2016
Tas, Emine Nakilcioglu | Otles, Semih
In this study, using an eco–friendly extraction system, it is aimed to obtained extracthas high polyphenol and organic acid content from black grape seeds which are one ofthe most important industrial waste for wine and grape juice. For this purpose grape seedextract was obtained by using pressurized water extractor on pilot scale at 50 bar, 50°Cand 90 min and its polyphenol and organic acid content was determined bychromatographic and spectroscopic methods. Total phenolic content was found as49.77% (w/w) in terms of gallic acid equivalent and antioxidant activity that was determined by DPPH method was 90.90% as inhibition, 98.13% as antiradical activity.As a result of high performance liquid chromatography (HPLC) analysis, tartaric, oxalic,L–malic acid and fumaric acid were found and the presence of hydroxytyrosol and (+)–catechin as polyphenol were revealed in extract. Grape seed extract is a commerciallyavailable product in the market. The extract obtained by the pressurized water extractionmethod, has a strong aroma and desirable stability with organic acids in the structure aswell as the rich of polyphenols content. Thus, grape seed extract which is a higher qualityand more prominent health effects will be possible to put on the market with the supportof eco–friendly extraction method used.
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