Isolation of methanol-assimilating Candida boidinii YF-3 and production of single cell protein
1987
Lee, K.H. | Bae, S.M. (Seoul National Univ., Suwon (Korea R.). Dept. of Food Science and Technology)
A large number of methanol-assimilating yeasts and bacteria were isolated from samples of soil, sewage, decomposed milk and spoiled sweet-radish pickles. Among the yeasts, one strain was selected and identified as a strain of Candida boidinii. In 1 % (v/v) methanol Candida boidinii YF-3 grew well and could grow in as much as 5 %. This yeast required biotin for growth. Maximum growth was observed at 30 deg C and pH 6 in a semisynthetic medium. The productivity was 2.72g of dry cells per liter in batch culture with 1 % (v/v) methanol and the cell yield for methanol was 0.39 gg** (-1). The specific growth rate was 0.11 h** (-1) and the generation time was 6.4 hours. The protein content of the cell was 45.5 % and total nucleic acid content was 5.9 %. The amino acid profile was as good as FAO standard for food protein
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