Detection and determination of aflatoxins in maize
1987
Wilson, D.M. (Georgia Univ., Tifton (USA) | Coastal Plain Experiment Station)
Many different methods are available for aflatoxin analysis. Maize is often contaminated with a combination of aflatoxins B1 and B2 and less often with a combination of B1, B2, G1 and G2. Safe handling of analytical samples in laboratories working with the aflatoxins or with the aflatoxigen1978ic species of the Aspergillus group is essential. Common methods for screening maize for aflatoxin contamination include the BGYF (bright greenish-yellow fluorescence) presumptive test and any one of several minicolumn methods. These methods should not be used to measure quantities of aflatoxin, but are effective for identifying lots of maize requiring further testing. Quantitative procedures for aflatoxins B1, B2, G1 and G2 include several thin-layer chromatographic (TLC) and high-performance liquid chromatographic (HPLC) methods. The present enzyme-linked immunosorbent assay (ELISA) methods are not sufficiently developed to be used for quantification, but they can be effectively calibrated to give positive results above a predetermined amount of aflatoxin B1. Current ELISA methods do not assess amounts of aflatoxin B2, G1 an
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