Comparison of agar and microcrystal cellulose as gelling agents for in vitro culture of Nicotiana tabacum stem explants
1993
Gorinova, N. | Atanasov, A. | Alexandrova, K. (Selskostopanska Akademiya, Sofia (Bulgaria)) | Velkova, R. | Kasachka, A.
Investigation was made on a use of microcrystal cellulose as a new and inexpensive gelling agent instead of agar. Microcrystal cellulose in concentration 20 % forms a suitable structure of nutrient medium for in vitro cultivation. The higher humidity in the culture container with microcrystal cellulose causes partial vitrification of Nicotiana tabacum plants, cv. Zlatna arda. It is proved by reduced chlorophyll content, changes in protein synthesis and strongly reduced isoenzyme spectrum of peroxidase.
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