Use of near-isogenic lines derived by backcrossing or selfing to map qualitative traits
1993
Kaeppler, S.M. (Minnesota Univ., St. Paul (USA). Dept. of Agronomy and Plant Genetics and the Plant Molecular Genetics Inst.) | Phillips, R.L. | Kim, T.S.
Near-isogenic lines (NILs) are a valuable resource for detecting linkages between qualitative trait loci and molecular markers. Molecular marker studies are expensive and methods that require genotyping fewer individuals, such as the NIL-analysis method, are desirable. A theory is presented for using sets of NILs to detect linkages between molecular markers and introgressed loci. The probability that a marker a specific distance from the introgressed gene will have a donor parent allele in a near-isogenic line is a function of the distance between the marker and the gene, and the number of backcrosses and/or selfs used in deriving the NIL. The binomial probability formula is used to calculate the probability of having a donor parent allele at a given marker when sets of NILs are used. The formulae given allow calculation of the probability that a marker is linked to the introgressed gene, as well as the probability that a gene will be successfully detected when using given numbers of NILs, backcrosses, and molecular markers.
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Эту запись предоставил ZB MED Nutrition. Environment. Agriculture