[Preliminary studies on the somaclonal selection system of chilli resistance to Colletotrichum capsici (Syd.) Butler and Bisby]
1994
Nurina Anuar (Kebangsaan Malaysia Univ., Bangi, Selangor (Malaysia). Jabatan Kejuruteraan Kimia dan Proses) | Ismail Ahmad | Azizah Hashim
Toxin treatment from the anthracnose pathogen, Colletotrichum capsici on chilli tissue culture was utilised as the selection mechanism for variants which are resistant to this pathogen. The crude toxin preparation was sterilised and then added to the MS medium at the concentration of 0, 10, 20, 30 or 40 (v/v). At concentrations higher than 20, the toxin prevented the formation of calli from hypocotyl explants, and at 10 and 20, the formation of calli was strongly inhibited. The calli whichsurvived the toxin treatment did not regenerate. Conversely, calli which were cultured on MS medium supplemented with V8 juice or on the control MS medium, were able to regenerate at 37.6 and 62.7, respectively. Induction of resistant variants was attempted by inoculating the calli which the conidia from the anthracnose pathogen. The antibiotic which had been extracted from an antagonistic fungus, Chaetomium trilaterale, was added to the MS medium to limit the spread of the pathogen to the medium from the chilli calli. From the antibiotic assay, the development of the pathogen was dependent on the antibiotic concentration used in the media. The minimum antibiotic concentration which successfully prevented the growth of the fungal pathogen was 100 mg/ml. At that concentration, the fungus was only observed on the calli but not on the medium. However, the calli turned brown after 2 weeks of inoculation. In addition, the calli that survived became compacted and failed to regenerate
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