Transient expression of heterologous PAL gene in the cells and tissues of muskmelon (Cucumis melo L. cv. Birdie)
1995
Norihan Mohd Saleh | Tan, S.H. | Ichinose, Y. | Yamada, T. (Pertanian Malaysia Univ., Serdang, Selangor (Malaysia). Dept. of Biotechnology)
Techniques to develop PAL gene expression in the cells and tissues of Cucumis melo L. were established using particle gun electroporation technique. These techniques enable us to study the regulation of PAL promoters, isolated from pea (Pisum sativum) in a heterologous plant system; pPaL1 and pPaL2 were shown to be expressible in melon. The effect of UV radiation on the regulation of the PAL promoters under different regime of UV irradiation was tested on the mesophyll protoplasts of melon. Maximum induction of GUS expression was observed in PSPAL1 and PSPAL2 at 2 min and 80 sec, respectively. Further confirmation of the GUS expression in the tissue of melon was carried out using particle. Similar pattern of induction of GUS expression by UV radiation has been regulated by the PSPAL1 and PSPAL2 promoter. Thus, this suggests that similar mechanism of PAL gene expression may be present in plant
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