The distribution of cDNA sequences on field bean chromosomes
1994
Houben, A. (Institut fur Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.) | Brandes, A. | Schubert, I.
Total polyadenylated RNA was isolated from different organs of the field bean by oligo-dT latex particles. Complementary DNA (cDNA) was reverse transcribed from the mRNAs and used as a probe for fluorescent in situ hybridization to metaphase chromosomes. Hybridization signals covered the whole chromosome complement except for late-replicating Giemsa-banded heterochromatin regions, which are composed mainly (or exclusively) of tandemly arranged repetitive sequences
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