The effectiveness of inactivated, purified and concentrated experimental rabies vaccine for veterinary use: immunogenic activity
1997
Sueliova, J. | Benisek, Z. | Svrcek, S. | Durove, A. | Ondrejka, R. (Univerzita Veterinarskeho Lekarstva, Kosice (Slovakia))
The authors prepared an experimental, inactivated, concentrated and highly purified rabies vaccine from the strain Vnukovo-32/107. The purification and concentration (simultaneous - in one operation) was carried out by affinity chromatography. The content of rabies antigen in the vaccine was 4.256 UE/cm3 (determined by ELISA method). The authors used their own lipoid adjuvant - type oil in water - to resolve the lyophilized vaccine to original volume. It is based on esters of fatty acids. The effectiveness - immunogenic activity - of the experimental preparation was compared with that of commercial rabies vaccines and the reference vaccine by means of the NIH [National Institute of Health] and NRLR [National Reference Laboratory of Rabies] methods. The results obtained (2.826 units/cm3 by the NIH and 3.085 units/cm3 by the NRLR method) confirmed that the method of affinity chromatography could by used to prepare a rabies vaccine, exhibiting effectiveness superior to commercial preparations available, and complying with the WHO criteria.
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