Induction of somaclonal variation by tissue culture and cytogenetic analysis in Oryza sativa
1998
Chatterjee, B. | Das Gupta, P. (Burdwan Univ. (India). Dept. of Botany)
Protocols were developed for plant regeneration from callus induced in mature embryos of rice. Somaclonal variation was scored by genome mutation, chromosome mutation and plasmon mutation in R0, R1 and R2 plant progenies. The frequency of haploids and diploids appeared in the ratio of 20:33. Variation in the chromosome number in callus cells was found to be high and age dependent. Different types of chlorophyll deficient mutants including albinos appeared in R2 plant progeny where gene mutation frequency was the highest (52.4 %). The results revealed that a high frequency of somaclonal variation is possible to generate by tissue culture techniques.
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