Cryopreservation of pollen of some species [of interest for agriculture and forestry]
1994
Negri, V. | Belardinelli, A. | Sorbolini, S. (Perugia Univ. (Italy). Istituto di Miglioramento Genetico Vegetale)
The genetic variability of a species is very important for its survival. In order to preserve the present genetic variability, gene banks are established where plants, seeds or pollen can be stored utilizing different techniques. Pollen can be deep-frozen at -196 deg C, but informations on cryopreservation of some species are lacking. Pollen from 25 species (apple, peaches, cherries, plums, horn chestnut, hornbean, beech, plane tree, oak, holm oak, maple, cornel, wistaria, buttercup, tulip, rose, iris, locust, Philadelphus coronarius, red bud, broom, sulla, sainfoin, white lupins, Medicago arborea) has been stored in liquid air (-180 deg C). The samples were dehydrated, frozen and stored; the variability of frozen pollen was tested by in vitro germination. Pollen from all the species, with the exception of pollen from some genotypes of hornbean, oak, holm, oak and maple, showed good viability after cryopreservation
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