Direct organogenesis in hypocotyl cultures of Tamarindus indica
1998
Jaiwal, S.P.K. | Gulati, A. | Dahiya, S. (M.D.Univ., Rohtak (India))
Direct differentiation of shoot buds from hypocotyl segments of 12-d-old seedlings of Tamarindus indica was obtained on MS medium with or without growth regulators. The highest regeneration (66 %) and the maximum number of shoots (3-4) per explant were obtained from the explants on MS medium containing 6-benzylaminopurine (5x10*[-6) M). A maximum roots per shoot were produced on medium containing 3-indole butyric acid (5x10*[-6) M). The resulting plantlets were hardened and transferred to soil in pots where 75 % of them survived and resumed growth. Histological examination of explants suggests that the shoots were of de novo origin which would make this system suitable for transformation experiments.
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