Potato virus S eradication by chemotherapy in vitro using ribavirin

Horackova, V.

Potato virus S eradication by chemotherapy in vitro using ribavirin

1998

Horackova, V. (Vyzkumny Ustav Bramborarsky, Havlickuv Brod (Czech Republic))

Potato virus S (PVS) was eradicated from systemically infected plants of eight potato hybrids and four potato varieties in culture in vitro: cultivation in a nutrient medium containing ribavirin, a synthetic riboside, was used. Virus concentration decreased gradually, after repeated subcultures of the top parts of plants to a ribavirin-containing medium. Effects of the concentration used, subculture number and treated variety were manifested. The highest number of virus S free regenerants was determined in media with ribavirin concentrations 0.002 and 0.003 %. Positive sanitation effects were observed from the second subculture in the separate varieties, the proportion of virus-free clones ranged from 55 to 100 % following the third subculture. No virus was detected by ELISA after regenerants were transplanted into greenhouse conditions. This method provides results comparable with those obtained by usual procedures of virus S eradication.

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