The distribution of enzymes in the kidneys of sheep fed a high and low protein diet
1998
Faixova, Z. | Levkut, M. | Kolodzieyski, L. (Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic)) | Faix, S. | Leng, L.
The distribution of 13 enzymes in the cortex, in the outer medulla and in the inner medulla of kidneys was investigated in sheep fed a high (HP) and low (LP) protein diet. Sheep on HP-diet were given a daily ration with 150.2 g of crude protein (CP) and 15.2 MJ of digestible energy (DE) while the daily intake of sheep on LP-diet was 58.3 g of CP and 14.2 MJ of DE. Both groups of animals were fed these diets for at least 6 weeks before the collection of kidneys. Histochemical analysis of enzymes showed that glucose-6-phosphatase and 5'-nucleotidase were not detectable in the kidney of sheep fed the HP-diet but they appeared in both the cortex and the outer stripe of the outer medulla of sheep on the LP-diet. Alkaline phosphatase of the inner stripe of the outer medulla and of the base of papilla was found in sheep fed the HP-diet only. Adenosine triphosphatase of the inner medulla appeared in sheep fed the LP-diet only. Changes in the distribution of other enzymes were only of a small magnitude. The present results demonstrate the enzyme expression of metabolic adaptation of sheep kidney to a low protein intake associated with an increased renal urea reabsorption.
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