Endogenous content of phytohormones and phenylpropanoids in sessile oak somatic embryos in relation to their conversion potential
2000
Mala, J. | Cvrckova, H. (Vyzkumny Ustav Lesniho Hospodarstvi a Myslivosti, Jiloviste-Strnady (Czech Republic)) | Brezinova, A. | Hrubcova, M. | Eder, J. | Vagner, M. | Cvikrova, M.
Two types of sessile oak (Quercus petraea) somatic embryos were examined for the endogenous contents of auxin (indole-3-acetic acid, IAA), abscisic acid (ABA), cytokinins (CKs), polyamines (PAs), phenolic acids (PhAs), lignin and peroxidase activity. The conversion into plantlets did not occur in embryos with deep green cotyledons (NCE, nonconverting embryos) while almost 56 % of embryos with ivory or light green cotyledons converted into plantlets (CE, converting embryos). High conversion capacity was associated with slightly higher content of IAA, lower content of ABA, markedly increased level of free isopentenyladenine and zeatin and their ribosides, lower levels of free putrescine and its soluble conjugates, and higher content of spermidine soluble conjugates, significantly lower content of total PhAs represented by the sum of free, soluble ester- and glycoside-, and insoluble cell wall-bound PhAs as compared to the contents in NCE. Higher levels of all determined free PhAs were found in NCE. On the contrary, three times higher ferulic acid content was found in the cell walls of CE. Higher content of lignin in NCE positively correlated with the amount of soluble phenolics and ionically bound peroxidase activity.
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