Selection of hydroxyproline-resistant cell lines from Solanum tuberosum L. callus. 1: Stability and frost tolerance
1998
Anjum M.A. | Villiers, T.A. (Salford Univ. (UK). Dept. of Biological Sciences)
Английский. Twenty three hydroxyproline (hyp) resistant lines were selected from callus cultures of S. tuberosum cvs. Desiree and Maris Piper by subculture on medium containing hydroxyproline (10, 20 or 30 mM). Ten lines were tested for their frost tolerance, nine were found to have increased degrees of frost tolerance. One of the two lines with high level of frost tolerance that were transferred to hyp-free medium, was found to be stable, with the same level of frost tolerance and hyp resistance when returned to the hyp-containing medium. The frost tolerance level of the remaining line was decreased but was still high compared with that of the non-selected control
Показать больше [+] Меньше [-]итальянский. [Sono state selezionate ventitre' linee resistenti all'idrossiprolina (hyp) da colture di callo di S. tuberosum cv. Desiree e Maris Piper, mediante subcoltura effettuata su un substrato contenente idrossiprolina (10, 20 o 30 mM). Dieci linee sono state saggiate per la tolleranza al gelo, di queste nove hanno manifestato un incremento del livello di tolleranza. Una delle due linee con elevata resistenza al gelo, che erano state trapiantate su un substrato non contenente hyp, si e' dimostrata stabile, con il medesimo livello di tolleranza al gelo e di resistenza all'hyp quando riportata su substrato contenente hyp. Il livello di tolleranza al freddo dell'altra linea di cellule diminuiva, ma risultava ancora alto rispetto a quello del controllo non selezionato]
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