Simultaneous identification of ryanodine receptor 1 (RYR1) and estrogen receptor (ESR) genotypes with multiplex PCR-RFLP method in Polish Large White and Polish Landrace pigs
2002
Kaminski, S. | Rusc, A. | Wojtasik, K. (University of Warmia and Mazury, Olsztyn (Poland). Dept. of Animal Genetics)
A method allowing simultaneous genotyping of two loci: ryanodine receptor (RYR1) and estrogen receptor (ESR) is presented. In multiplex PCR amplification, two amplicons were simultaneously produced: a 272 bp fragment of RYR1 gene and were then subjected to "one-tube" restriction enzyme digestion with Hin6 I and Ava I, respectively. A total of 122 Polish Large White and Polish Landrace pigs were genotyped by this method, demostrating its reliability, covenience and lower costs. This method may be useful in the wide-scale genotyping of both loci in pig breeding programmes
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