HPLC Analysis, antimicrobial and antioxidant activities of Daphne cneorum L.
2012
Manojlović, Nedeljko T. (Medical Faculty, Kragujevac (Serbia)) | Mašković, Pavle Z. (Faculty of Agronomy, Čačak (Serbia)) | Vasiljević, Perica J. (Faculty of Sciences and Mathematics, Niš (Serbia)) | Jelić, Ratomir M. (Medical Faculty, Kragujevac (Serbia)) | Jusković, Marina Ž. (Faculty of Sciences and Mathematics, Niš (Serbia)) | Sovrlić, Miroslav (Medical Faculty, Kragujevac (Serbia)) | Mandić, Leka (Faculty of Agronomy, Čačak (Serbia)) | Radojković, Marija (Faculty of Technology, Novi Sad (Serbia))
The present study describes in vitro antimicrobial and antioxidant activities of methanol extracts obtained from the leaves and twigs of the plant Daphne cneorum L. The antimicrobial activity of these extracts was tested against human pathogenic microorganisms using a minimum inhibitory concentration (MIC) values. Total phenolics and flavonoids, as well as the antioxidant activity of the extracts were determined. The two tested extracts showed good antimicrobial and antioxidant activities. The results of a high performance liquid chromatographic (HPLC) method showed that 7,8-dihydroxycoumarin is one of the most abundant secondary metabolite in the tested extracts. The results of this study clearly indicated that the extracts of D. cneorum could be used as a potential source of natural antioxidant and antimicrobial agents.
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