Start-up phase of an anaerobic full-scale farm reactor – Appearance of mesophilic anaerobic conditions and establishment of the methanogenic microbial community

Goux, Xavier; Calusinska, Magdalena; Fossépré, Marie; Benizri, Émile; Delfosse, Philippe

Start-up phase of an anaerobic full-scale farm reactor – Appearance of mesophilic anaerobic conditions and establishment of the methanogenic microbial community

2016

Goux, Xavier | Calusinska, Magdalena | Fossépré, Marie | Benizri, Émile | Delfosse, Philippe

The goal of this study was to investigate how the microbial community structure establishes during thestart-up phase of a full-scale farm anaerobic reactor inoculated with stale and cold cattle slurry. The16S/18S high-throughput amplicon sequencing results showed an increase of the bacterial, archaealand eukaryotic diversity, evenness and richness during the settlement of the mesophilic anaerobic conditions.When a steady performing digestion process was reached, the microbial diversity, evenness andrichness decreased, indicating the establishment of a few dominant microbial populations, best adaptedto biogas production. Interestingly, among the environmental parameters, the temperature, alkalinity,free-NH3, total solids and O2 content were found to be the main drivers of microbial dynamics.Interactions between eukaryotes, characterized by a high number of unknown organisms, and the bacterialand archaeal communities were also evidenced, suggesting that eukaryotes might play importantroles in the anaerobic digestion process.

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