Differential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit.
ALVES, G. S. C. | TORRES, L. F. | DÉCHAMP, E. | BREITLER, J-C. | JOËT, T. | GATINEAU, F. | ANDRADE, A. C. | BERTRAND, B. | MARRACCINI, P. | ETIENNE, H. | Gabriel Sergio Costa Alves, Universidade Federal de Lavras.; Luana Ferreira Torres, Universidade Federal de Lavras; Eveline Déchamp, CIRAD, UMR IPME; Jean-Christophe Breitler, CIRAD, UMR IPME; Thierry Joët, IRD, UMR DIADE; Frédéric Gatineau, CIRAD, UMR AGAP; ALAN CARVALHO ANDRADE, SAPC; Benoît Bertrand, CIRAD, UMR IPME; Pierre Marraccini, CIRAD, UMR AGAP; Hervé Etienne, CIRAD, UMR IPME.
Despite the importance of the DREB1D gene (also known as CBF4) in plant responses to water deficit and cold stress, studies analysing its regulation by transgenic approaches are lacking. In the current work, a functional study of three CcDREB1D promoter haplotypes (named HP15, HP16 and HP17) isolated from drought-tolerant and droughtsensitive clones of Coffea canephora was carried out in plants of C. arabica stably transformed by Agrobacterium tumefaciens by analysing their ability to regulate the expression of the uidA reporter gene in response to water deficit mimicked by polyethylene glycol (−2.0 MPa) and low relative humidity treatments.
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