Survival of Candida tropicalis and Lactobacillus plantarum starter culture after using protective agent and drying
2018
Masniyom, P. | Maneesri, J. | 1 Liming, M.
Yeast Candida tropicalis and lactic acid bacteria Lactobacillus plantarum were isolated from Ka-nom Tuay-fu as traditional starter culture in Thailand. The isolates were used on a labolaratory-scale process. Effect of temperature drying (40, 50 and 60ºC) on the survival and activity of C. tropicalis and L. plantarum were investigated. Rice flour was used as supporting material. The temperature drying had affect cell viability, moisture content, water activity and drying time. The cell viability of starter cultures have significantly affect under different temperature. The dry condition at 40ºC for 16 hr was obtained the high cell viability of C. tropicalis and L. plantarum with 7x106 and 5x106 CFU/5g, respectively. The starter culture powder can dehydrated to low of the moisture content at 10% and water activity 0.4. Sucrose and glucose solution were used as protective agent at concentrations of 0-15%(w/v). Glucose 15%(w/v) showed superior protective on recovery cell survival of C. tropicalis and L. plantarum up to 99 and 90%, respectively. The result revealed the optimal condition of drying process can be used for preparation of the Ka-nom Tuay-fu starter culture.
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