Anti-inflammatory activity of essential oils from Tunisian aromatic and medicinal plants and their major constituents in THP-1 macrophages
2023
Pereira, Renato, B | Rahali, Fatma, Zohra | Nehme, Ralph | Falleh, Hanen | Jemaa, Mariem Ben | Sellami, Ibtissem Hamrouni | Ksouri, Riadh | Bouhallab, Said | Ceciliani, Fabrizio | Abdennebi-Najar, Latifa | Pereira, David, M | REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, n° 228, 4050-313 Porto | Laboratory of Aromatic and Medicinal Plants, Biotechnology Center of Borj-Cédria, BP 901, 2050 Hammam-Lif, Tunisia | Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) | Quality and Health Department, IDELE Institute, 149 rue de Bercy, 75595 Paris Cedex 12 | Department of Veterinary Medicine, Università degli Studi di Milano, 20122 Milano | Centre de Recherche Saint-Antoine (CRSA) ; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU) | This work was funded by EU-PRIMA (H2020-Prima 2018 – Section 2, Project MILKQUA), the Foundation for Science and Technology (FCT, Portugal) and FEDERCOMPETE-QREN-EU for financial support to theresearch centers REQUIMTE (UIDB/50006/2020 and UIDP/50006/ 2020). Renato B. Pereira acknowledges PRIMA Foundation (PRIMA/ 0007/2018) and FCT (PTDC/QUI-QFI/2870/2020) for the funding | https://www.sciencedirect.com/science/article/pii/S0963996923002235
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Показать больше [+] Меньше [-]Английский. In this study, the capacity of eight essential oils (EOs), sage (Salvia officinalis), coriander (Coriandrum sativum), rosemary (Rosmarinus officinalis), black cumin (Nigella sativa), prickly juniper (Juniperus oxycedrus), geranium (Pelargonium graveolens), oregano (Origanum vulgare) and wormwood (Artemisia herba-alba), on the inhibition of NF-κB activation was screened at concentrations up to 0.25 µL/mL using THP-1 human macrophages bearing a NF-κB reporter. This screening selected coriander, geranium, and wormwood EOs as the most active, which later evidenced the ability to decrease over 50 % IL-6, IL-1β, TNF-α and COX-2 mRNA expression in LPS-stimulated THP-1 macrophages. The chemical composition of selected EOs was performed by gas chromatography-mass spectrometry (GC-MS). The two major constituents (>50 % of each EO) were tested at the same concentrations presented in each EO. It was demonstrated that the major compound or the binary mixtures of the two major compounds could explain the anti-inflammatory effects reported for the crude EOs. Additionally, the selected EOs also inhibit>50 % caspase-1 activity. However, this effect could not be attributed to the major components (except for β-citronellol/geranium oil, 40 %/65 % caspase-1 inhibition), suggesting, in addition to potential synergistic effects, the presence of minor compounds with caspase-1 inhibitory activity. These results demonstrated the potential use of the EOs obtained from Tunisian flora as valuable sources of anti-inflammatory agents providing beneficial health effects by reducing the levels of inflammatory mediators involved in the genesis of several diseases.
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