Down Regulation and Loss of Auxin Response Factor 4 Function Using CRISPR/Cas9 Alters Plant Growth, Stomatal Function and Improves Tomato Tolerance to Salinity and Osmotic Stress
2020
Bouzroud, Sarah | Gasparini, Karla | Hu, Guojian | Barbosa, Maria Antonia Machado | Rosa, Bruno Luan | Fahr, Mouna | Bendaou, Najib | Bouzayen, Mondher | Zsögön, Agustin | Smouni, Abdelaziz | Zouine, Mohamed | Laboratoire de Physiologie et Biotechnologie Végétale ; Université Mohammed V de Rabat [Agdal] (UM5) | Génomique et Biotechnologie des Fruits (GBF) ; École nationale supérieure agronomique de Toulouse (ENSAT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Universidade Federal de Viçosa = Federal University of Viçosa (UFV) | Hubert-Curien partnership program Volubilis {MA/12/280-27105PL} | ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011) | ANR-16-CE20-0014,TomEpiSet,La parthénocarpie comme une stratégie pour surmonter la baisse de nouaison et du rendement en fruits chez la tomate dans des conditions de stress thermique(2016)
Auxin controls multiple aspects of plant growth and development. However, its role in stress responses remains poorly understood. Auxin acts on the transcriptional regulation of target genes, mainly through Auxin Response Factors (ARF). This study focuses on the involvement of SlARF4 in tomato tolerance to salinity and osmotic stress. Using a reverse genetic approach, we found that the antisense down-regulation of SlARF4 promotes root development and density, increases soluble sugars content and maintains chlorophyll content at high levels under stress conditions. Furthermore, ARF4-as displayed higher tolerance to salt and osmotic stress through reduced stomatal conductance coupled with increased leaf relative water content and Abscisic acid (ABA) content under normal and stressful conditions. This increase in ABA content was correlated with the activation of ABA biosynthesis genes and the repression of ABA catabolism genes. Cu/ZnSOD and mdhar genes were up-regulated in ARF4-as plants which can result in a better tolerance to salt and osmotic stress. A CRISPR/Cas9 induced SlARF4 mutant showed similar growth and stomatal responses as ARF4-as plants, which suggest that arf4-cr can tolerate salt and osmotic stresses. Our data support the involvement of ARF4 as a key factor in tomato tolerance to salt and osmotic stresses and confirm the use of CRISPR technology as an efficient tool for functional reverse genetics studies.
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