Two plastidial lysophosphatidic acid acyltransferases differentially mediate the biosynthesis of membrane lipids and triacylglycerols in Phaeodactylum tricornutum
2023
You, Lingjie | Połońska, Ada | Jasieniecka-Gazarkiewicz, Katarzyna | Richard, Fabien | Jouhet, Juliette | Maréchal, Eric | Banaś, Antoni | Hu, Hanhua | Pan, Yufang | Hao, Xiahui | Jin, Hu | Allen, Andrew | Amato, Alberto | Gong, Yangmin | Oil Crops Research Institute (OCRI) ; Chinese Academy of Agricultural Sciences (CAAS) | University of Gdańsk (UG) | Medical University of Gdańsk | LIPID ; Physiologie cellulaire et végétale (LPCV) ; Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) ; Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) ; Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA) | Institute of Hydrobiology [Wuhan] ; Chinese Academy of Sciences [Beijing] (CAS) | Scripps Institution of Oceanography (SIO - UC San Diego) ; University of California [San Diego] (UC San Diego) ; University of California (UC)-University of California (UC) | Grant from the National Natural Science Foundation of China (31961133008) | International Partnership Program of Chinese Academy of Sciences (Grant No. 075GJHZ2022014MI) | National Science Center (NCN, Poland; No.UMO-2018/30/Q/NZ3/00497) | Grants from the United States Department of Energy Genomics Science program | US National Science Foundation, NSF-MCB-1818390 | Gordon and Betty Moore Foundation GBMF3828 | ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010) | ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011) | ANR-15-IDEX-0002,UGA,IDEX UGA(2015)
International audience
Показать больше [+] Меньше [-]Английский. Lysophosphatidic acid acyltransferases (LPAATs) catalyze the formation of phosphatidic acid (PA), a central metabolite in both prokaryotic and eukaryotic organisms for glycerolipid biosynthesis. Phaeodactylum tricornutum contains at least two plastid‐localized LPAATs (ptATS2a and ptATS2b), but their roles in lipid synthesis remain unknown. Both ptATS2a and ptATS2b could complement the high temperature sensitivity of the bacterial plsC mutant deficient in LPAAT. In vitro enzyme assays showed that they prefer lysophosphatidic acid over other lysophospholipids. ptATS2a is localized in the plastid inner envelope membrane and CRISPR/Cas9‐generated ptATS2a mutants showed compromised cell growth, significantly changed plastid and extra‐plastidial membrane lipids at nitrogen‐replete condition and reduced triacylglycerols (TAGs) under nitrogen‐depleted condition. ptATS2b is localized in thylakoid membranes and its knockout led to reduced growth rate and TAG content but slightly altered molecular composition of membrane lipids. The changes in glycerolipid profiles are consistent with the role of both LPAATs in the sn ‐2 acylation of sn ‐1‐acyl‐glycerol‐3‐phosphate substrates harboring 20:5 at the sn ‐1 position. Our findings suggest that both LPAATs are important for membrane lipids and TAG biosynthesis in P. tricornutum and further highlight that 20:5‐Lyso‐PA is likely involved in the massive import of 20:5 back to the plastid to feed plastid glycerolipid syntheses.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Institut national de la recherche agronomique