Design of experimental approach for maximal extraction of vitamin D2 from mushrooms
2020
Shaikh, Eram | Khare, Lalit | Jain, Ratnesh | Dandekar, Prajakta
Vitamin D2 is the standard form of fortification and supplementation of food in most countries because of its good bioavailability and its ability to substitute vitamin D3. In this study, tetrabutylammonium bromide/decanoic acid-based hydrophobic deep eutectic solvent was employed for green extraction of ergosterol from mushrooms. Extraction factors, stirring time, sonication time, solvent volume and solvent ratio were optimized by response surface methodology to attain maximum extraction yield of 3 900 mg ergosterol per kilogram dry weight mushrooms. The extract was purified using preparative high performance liquid chromatography (HPLC). The extracted ergosterol was subjected to ultravoilet radiation and maximum ergocalciferol yield of 1 290.3 mg·kg-1 dry weight mushroom was obtained. This study also describes a HPLC method for analysing the stability of Vitamin D2 and its validation using the International Council for Harmonisation guidelines. The method was optimized exposing a standard ergocalciferol solution to various stress conditions. The degradation products formed during initial elucidation by HPLC were further analysed by electrospray ionization mass spectrometry.
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