Detection of adulterations in a valuable Brazilian honey by using spectrofluorimetry and multiway classification
Antônio, Daphne Chiara | De Assis, Débora Cristina Sampaio | Botelho, Bruno Gonçalves | Sena, Marcelo Martins
Distinguishing floral and honeydew honey using chemical analysis is generally problematic. This work solved this problem by determining the trisaccharide melezitose in honeys, which was identified as a potential marker for identification of the type of honey. The newly developed method for determination of melezitose reduces the analysis time by approximately 10 times and significantly simplifies sample preparation during derivatization of saccharides. A two-step procedure of silylation with hexamethyldisilazane followed by N,O-bis(trimethylsilyl)trifluoroacetamide was developed for derivatization without the formation of degradation products. The limit of detection of the method for melezitose was 4.9 mg·kg-1 based on the selected ion m/z of 361 with relative standard deviation for real samples of ≤ 15 %. The simplicity and speed of the method enables its use in screening tests. The method was successful at determination of a significant difference in the melezitose content of floral (103–2 880 mg·kg-1) and honeydew honeys (10 600–26 100 mg·kg-1), which allowed differentiation between the types of honey.
Показать больше [+] Меньше [-]