Optimization of Recombinant Glucagon-like Peptide-1 Production Using Recombinant E. coli
2023
Jeon, Y.C. | Kwon, D.H. | Lee, Y.J. | Lee, S.H. | Kwon, S.H. | Jung, H.M. | Ha, S.J.
Obesity is well known as a metabolic abnormality and potentially life-threatening condition associated with several chronic diseases. Various peptide drugs have been developed to treat obesity. In this study, optimization of expression conditions were performed to produce glucagon-like peptide1 (GLP-1), which is used as an obesity treatment in recombinant E. coli. After the construction of expression vector system and optimization of expression conditions, the productivity of GLP-1 was increased to 11.18 mg/L·h in flask cultivation. Through fermentation conditions were optimized for bioreactor, the GLP-1 productivity was increased to 16.80 mg/L·h in bioreactor, which was 50.27% improvement as compared to the flask cultivation. When 1.00 mM lactose was used as an inducer instead of IPTG, 17.39% higher expression was shown than that with 0.01 mM IPTG.
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