Determination of PrP locus SNPs in Garole sheep breed for scrapie
2013
Choudhary, Sharda | Ahlawat, S.P.S. | Gupta, S.C. | Guptā, Nīlama
Experiment was carried out at National Bureau of Animal Genetic Resources, Karnal to identify polymorphisms of the prion protein gene (PrP) at the codons (136, 154 and 171) responsible for the susceptibility and resistance of the scrapie disease in the sheep. Blood samples of 50 animals of the Garole sheep breed were collected from its native tract in and around South 24 Pargana district of West Bengal from the eastern region. PCR amplicons of approximate size 358 bp were generated for PrP gene exon-3 partial CD's covering 352 to 700 nucleotide positions. Genotype ARQ/ARQ was found in the analyzed population with the 100% frequency. SNP at codon 127 and 189 are GGIGS and QQ/QL with frequencies of 72.73/27.27 and 90.91/9.09 respectively. In all flocks, ARQ/ARQ genotype was the dominant haplotype regardless of sheep age, sex and health. However, the generated data will help for the detection of SNP markers related to disease resistance or susceptibility in India.
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