Radio-decontamination efficacy and safety studies on optimized decontamination lotion formulation
2012
Rana, S. | Bhatt, S. | Dutta, M. | Khan, A.W. | Kaẓmī, Jān ʻAlī Shāh | Sultana, Sabnam | Kotta, S. | Ansari, S.H. | Sharma, R.K.
Objective of the present study was to optimize decontamination lotion and to evaluate its relative decontamination efficacy using three radio-isotopes (Technetium-99m, Iodine-131 and Thallium-201) as contaminants with varying length of contaminant exposure (0–1h). Experiments were performed on Sprague Dawley rat's intact skin and human tissue equivalent models. Rat's hair was removed by using depilator after trimming with scissors. Relative decontamination efficacy of the optimized lotion was investigated and compared with water as control. Static counts were recorded before and after decontamination using single photon emission computed tomography (SPECT). Measured decontamination efficacy (DE) values were analyzed using one way ANOVA and Student's t-test (p value<0.05) and were found statistically significant. Decontamination efficacy of the lotion was observed to be 90±5%, 80±2% and 85±2%, for the ¹³¹I, ²⁰¹Tl and ⁹⁹ᵐTc radio-contaminants respectively on skin. Reduced contaminant removal was recorded for the skin which was cleaned by depilator (50–60%). Skin decontamination was found more efficacious for rat skin decontamination than the human tissue equivalent model. Decontamination efficacy of the lotion against ⁹⁹ᵐTc was recorded 70±15% at 0–1h on the tissue equivalent model. In vitro chelation efficacy of the lotion was also established by using the instant thin layer chromatography-slica gel (ITLC-SG) and >95% of ⁹⁹ᵐTc was recorded. Neither erythema nor edema was scored in the primary skin irritancy test visually observed for two weeks.
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