Halobellus captivus sp. nov., an extremely halophilic archaeon isolated from a subterranean salt mine
2020
Chen, Shaoxing | Sun, Siqi | Xu, Yao | Chen, Feilong | Liu, Jingwen
An extremely halophilic archaeon, strain ZY21ᵀ, was isolated from a subterranean rock salt sample in Yunnan, China. Colonies of strain ZY21ᵀ on nutrient-rich agar plates are orange, wet and transparent. Cells are pleomorphic, motile, Gram-stain negative and lyse in distilled water. Cells can grow at 20–55 °C (optimum 42 °C), in the presence of 15–30% (w/v) NaCl (optimum 18–20%) and at pH 6.0–9.5 (optimum 7.5). Mg²⁺ is required for growth (optimum 0.3 M). The major polar lipids of strain ZY21ᵀ are phosphatidylglycerol, phosphatidylglycerol sulfate and phosphatidylglycerol phosphate methyl ester, sulfated mannosyl-glucosyl-glycerol diether-1 and seven unidentified glycolipids. Sequence similarity searches with the 16S rRNA gene and rpoB′ gene showed that strain ZY21ᵀ is closely related to Halobellus rufus CBA1103ᵀ (sequence similarities: 97.5% for 16S rRNA gene and 93.3% for rpoB′ gene). The DNA G+C content of strain ZY21ᵀ was determined to be 63.0 mol% based on the draft genome sequence. Genome-based sequence similarity analysis showed that the values of the ANI, AAI, and DDH were far below the boundary for delineation of new species. Phenotypic, chemotaxonomic characteristics and phylogenetic properties suggest that strain ZY21ᵀ represents a novel species in the genus Halobellus, for which the name Halobellus captivus sp. nov. is proposed. The type strain is ZY21ᵀ (= CGMCC 1.16343ᵀ = NBRC 113439ᵀ).
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