Monitoring Simulated Bioflocculation: Application of Micro-Flow Imaging Technology

Kollu, Kerim; Örmeci, Banu

Monitoring Simulated Bioflocculation: Application of Micro-Flow Imaging Technology

2015

Kollu, Kerim | Örmeci, Banu

Bioflocculation occurs in both engineered and natural systems and plays an important role in several water treatment processes as well as in pathogen transport and survival. In this study, bioflocculation was simulated in the laboratory to allow for well-controlled experiments. Escherichia coli and latex particles of varying sizes (3.2, 11 and 25 μm) were spiked into a buffer solution and were bioflocculated by adding alginate and varying amounts of calcium (0, 5, 10 and 15 mM). The extent of flocculation was determined by the calcium concentration, and the floc structure was modified by varying the particle size. The bioflocculation process was monitored with a dynamic particle analyzer, and the flocs formed were analyzed with respect to size, shape and porosity parameters. Larger flocs were observed to have a more heterogeneous structure with higher variation in shape and porosity compared to smaller flocs. Circularity and porosity parameters were shown to be strongly correlated with the calcium concentration. In addition, ultraviolet (UV) irradiation experiments were performed on flocculated and non-flocculated samples, and the inactivation data were assessed in light of floc characteristics determined with the particle analyzer.

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