Identification of tetrodotoxin-producing bacteria from goby Yongeichthys criniger
2015
Wei, Fen | Ma, Tinglong | Gong, Xiaoling | Zhang, Na | Bao, Baolong
Toxic goby fish (Yongeichthys criniger) containing tetrodotoxin (TTX), from Zhanjiang, Guangdong province, China, were screened for TTX-producing bacteria. Two toxic bacterial strains were isolated from the liver of Y. criniger and respectively denoted XC3-3 and XL-1. TTX production by the strains was confirmed by mouse bioassay, enzyme-linked immunosorbent assay and high performance liquid chromatography coupled with mass spectrometry. Based on morphological, physiological and biochemical characteristics and 16S rDNA phylogenetic analysis, strain XC3-3 was identified as Enterobacter cloaca and XL-1 was closely related to Rahnella aquatilis. These findings show for the first time that TTX-producing bacteria are symbiotic bacteria in goby and suggest that bacterial strains are at least partially responsible for TTX accumulation in Y. criniger.
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