Identification and molecular characterisation of fifteen tropical isolates of the entomopathogenic fungus Metarhizium
Castro-Vásquez, R. M. | Hernández-Villalobos, S. | Montero-Astúa, M. | Vargas-Martínez, A. | González-Herrera, A. | Molina-Bravo, R.
Twelve Metarhizium isolates from Costa Rica, two from Honduras and one from Nicaragua were characterised using molecular and morphological information. Two gene sequences, TEF1-α and β-TUB, identified isolates as M. anisopliae, M. brunneum and M. robertsii, and has provided new sequence information. Eleven SSRs revealed fourteen different genotypes. Morphological variability coincided with allelic diversity within species. This work provides a starting point for the application of integral biocontrol programmes, but more comprehensive and detailed surveys are warranted to understand the species composition and genetic diversity of the Metarhizium in Central America.
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