Plantlet regeneration via somatic embryogenesis in anther callus of Vitis latifolia L

Salunkhe, C.K.; Rao, P.S.; Mhatre, M.

Plantlet regeneration via somatic embryogenesis in anther callus of Vitis latifolia L

1999

Salunkhe, C.K. | Rao, P.S. | Mhatre, M.

Anthers of Vitis latifolia L.(wild grape) cultured on Nitsch and Nitsch medium supplemented with 20 micromolar 2,4-D and 9 micromolar BAP produced callus after 4-6 weeks. Subculture of callus onto Nitsch and Nitsch medium containing 10 micromolar NAA produced somatic embryos within 6 weeks. On growth regulator-free Nitsch and Nitsch basal medium somatic embryos converted to plantlets in 6-8 weeks. One gram of callus produced more than 400 somatic embryos with 13.7% being converted to complete plantlets, which were subsequently established in soil. Regenerated plants were found to have mixoploid populations of cells, 2n = 38 and n = 19.

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