Enhanced ajmalicine production in Catharanthus roseus cell cultures by combined elicitor treatment: from shake-flask to 20-1 airlift bioreactor
2000
Zhao, J. | Zhu, W.H. | Hu, Q.
A two-stage process for enhanced ajmalicine production in elicited Catharanthus roseus cell cultures was developed in shake-flasks and a bioreactor. By using combined elicitor treatment of an Aspergillum niger mycelium and tetramethyl ammonium bromide, yields of ajmalicine were 48 mg l-1, 52 mg l-1 and 33 mg l-1, respectively in 500-ml flasks, 1000-ml flasks and a 20-l airlift bioreactor. The peroxidase and superoxide dismutase activities decreased in elicited cell cultures but catalase and lipoxygenase activities increased in these cultures. The combined elicitor treatment also caused a significant increase of malondialdehyde content in cell cultures.
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