Decolorization of Textile Effluent by Soluble Fenugreek (Trigonella foenum-graecum L) Seeds Peroxidase

Husain, Qayyum; Karim, Zoheb; Banday, Zeeshan Zahoor

Decolorization of Textile Effluent by Soluble Fenugreek (Trigonella foenum-graecum L) Seeds Peroxidase

2010

Husain, Qayyum | Karim, Zoheb | Banday, Zeeshan Zahoor

Peroxidase from fenugreek (Trigonella foenum-graecum) seeds was highly effective in the decolorization of textile effluent. Effluent was recalcitrant to the action of fenugreek seeds peroxidase (FSP). However, in order to effectively decolorize effluent by peroxidase, the role of six redox mediators has been investigated. The maximum decolorization of textile effluent was observed in the presence of 1.0 mM 1-hydroxybenzotrizole, 0.7 mM H₂O₂, and 0.4 U ml⁻¹ of FSP in the buffer of pH 5.0 at 40°C in 2.5 h. The decolorization of textile effluent in a batch process by peroxidase was 85% in 5 h, whereas the complete decolorization of textile effluent by membrane-entrapped FSP was observed within 11 h of its operation. The absorption spectra of treated effluent exhibited a marked diminution in the absorbance at different wavelengths compared to untreated effluent.

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