Laccase-based biosensor for determination of polyphenols: determination of catechols in tea
1992
Ghindilis, A.L. | Gavrilova, V.P. | Yaropolov, A.I.
A new method of amperometric determination of phenolic compounds using an enzyme electrode is proposed. The latter represents the combination of the oxygen electrode and immobilized laccase. Analytical systems of flow injection and batch types were considered. A method of immobilization was developed that provided an increase in the stablity of the enzyme. Optimal conditions for biosensor operation were found. The time needed for analysis in the flow injection mode was below 100 s. A column with immobilized enzyme could be used for up to 500 determinations of phenolic compounds without decrease of the enzyme activity. The practical validity of the method was demonstrated by tanin analysis in tea of different brands.
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