Microalgal photosynthetic activity measurement system for rapid toxicity assessment
2008
Cho, Chul-Woong | Pham, Thi Phuong Thuy | Jeon, You-Chul | Min, Jiho | Jung, Ho-Young | Lee, Dae Sung | Yun, Yeoung-Sang
A systematic biosensor is constructed for the estimation of toxic compounds based on photosynthetic activity measurement in Selenastrum capricornutum cells. The photosynthetic response was evaluated as a function of light intensity, cell concentration and initial dissolved oxygen. The inhibitory effect of some toxicants (1-butyl-3-methylimidazolium tetrafluoroborate, methanol) on dissolved oxygen production was also determined. In all cases, a toxic response was detected (i.e. a dose-related inhibition of photosynthetic activity was observed). For the present system, a time of only 2 h was needed to predict EC50 values as compared to 96 h for a conventional algal assay based on algal growth rate. Thus, the developed biosensor was proved to be useful as a rapid and simple test method in environmental toxicity assessment.
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