Facile Approach To Observe and Quantify the αIIbβ3 Integrin on a Single-Cell
2015
Zhai, Jiao | Wang, Yaling | Xu, Chao | Zheng, Lingna | Wang, Meng | Feng, Weiyue | Gao, Liang | Zhao, Lina | Liu, Ru | Gao, Fuping | Zhao, Yuliang | Chai, Zhifang | Gao, Xueyun
We report for the first time seeing and counting integrin αIIbβ₃ on a single-cell level. The proposed method is based on the using of the Au cluster probe. With the fluorescent property of Au₂₄ cluster and the specific targeting ability of peptide, our probe can directly visualize integrin αIIbβ₃ on the membrane of human erythroleukemia cells (HEL) via confocal microscopy. On the basis of the accurate formula of our probe (Au₂₄Peptide₈), the number of integrin αIIbβ₃ can be precisely counted by quantifying the gold content on a single HEL cell via laser ablation inductively coupled plasma mass spectrometry. Our results reveal that the number of integrin αIIbβ₃ on a single cell varies from 5.75 × 10–¹⁷ to 9.11 × 10–¹⁷ mol, because of the heteroexpression levels of αIIbβ₃ on individual cells.
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