A new amplified fluorescent aptasensor based on hairpin structure of G-quadruplex oligonucleotide-Aptamer chimera and silica nanoparticles for sensitive detection of aflatoxin B1 in the grape juice
2018
Taghdisi, Seyed Mohammad | Danesh, Noor Mohammad | Ramaz̤ānī, Muḥammad | Abnous, Khalil
As one of the most toxic mycotoxins, aflatoxin B₁ (AFB₁) is a major food pollutant which can pose a high risk to human health. In this work, an accurate fluorescent sensing method was proposed for AFB₁ determination, based on hairpin structure of G-quadruplex oligonucleotide-Aptamer chimera, silica nanoparticles coated with streptavidin (SNPs-Streptavidin) and N-methyl mesoporphyrin IX (NMM). The hairpin structure of chimera and SNPs-Streptavidin allowed AFB₁ detection with high sensitivity and specificity. Moreover, the developed sensor could detect AFB₁ in 30 min. The relative fluorescence intensity increased as AFB₁ concentrations increased with a linear range of 30–900 pg/mL and a limit of detection (LOD) of 8 pg/mL. The constructed aptasensor was successfully employed to assess AFB₁ spiked grape juice and human serum samples. The analytical recovery of AFB₁ in the grape juice samples ranged from 95 to 106%, implying the great potential of the presented aptasensor in food product analysis.
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